CD19 cell surface antigen, a 96kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells.
B cells can be detected in peripheral blood and tissues.
Labels all B lymphocytes in peripheral blood and spleen but is negative on granulocytes, monocytes, platelets, T lymphocytes and erythrocytes.
This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1:50-1:100Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes human CD19.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to human CD19 at C-terminal (KLH).