Monoclonal antibodies clustered as CD3 recognize the constant structure of the CD3/TcR (T cell receptor) complex.
This complex is present on mature T cells and during thymopoiesis.
CD3/TcR is called the T cell receptor complex.
This complex is composed of different structures, i.e.
TcRa, TcRb, TcRg, TcRd, CD3g, CD3d, CD3e, the x-chain, and the h-chain.
Less than 10% of human peripheral T cells express the g/d TcR complex.
The function of a/b and g/d heterodimers is the recognition of peptide antigen bound to MHC, followed by T cell activation.
Recent data suggest that CD3 chains are able to function autonomously in signal transduction.
T cells play a major role in activation processes of the immune system.
Detection of these cells is important during immune monitoring.
In addition, a large number of leukemias are T cell-related disorders, in which antibodies recognizing CD3/TcR complex are important diagnostic tools.
Differentiation between AML, T-ALL and B-ALL can be made by immunophenotyping with (early) differentiation markers.
Cell surface detection using CD3 antibodies can be applied for detection of mature T-ALL, while immature T-ALL express cytoplasmic (cyCD3) but no surface CD3.
CD3 antibodies are used, in characterization of various subtypes of chronic lymphoid leukemias.
Examples of these chronic T cell leukemias are T-CLL (Sezary Syndrome) and the peripheral T cell lymphoma (ATLL) which co-express CD2, CD3, CD4 and CD5 antigens.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity：Recognizes human CD3.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human CD3 at C-terminal (KLH).
