Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme.
Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively.
Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis.
Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Caspase 3. Species sequence homology: Bovine, chicken, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, yeast.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to full length human Caspase 3.
