BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
It may play a role in the postsynaptic responses of hippocampal neurons.
This cytoplasmic protein is expressed in brain and testis.
Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL).
This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Formalin fixed paraffin embedded): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human BRAF when phosphorylated at Thr598/Ser601.
Isotype：IgG
Calc Applications Abbrev：IF IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human BRAF (KLH) around the region of Thr598/Ser601.
