BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities.
BAX protein forms a heterodimer with BCL2, and functions as an apoptotic activator.
This protein is reported to interact with, and increase the opening of, the mitochondrial voltage- dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c.
The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 0.2% gelatin.
Specificity：Recognizes mouse BAX. Species sequence homology: Human and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to mouse BAX at N-terminal (KLH).
