Beta galactosidase is an inducible enzyme that catalyses the hydrolysis of lactose and other betagalactosides into monosaccharides.
Beta galactosidase is a product of the LacZ operon, and is a homotetrameric protein consisting of four identical subunits of ~116kD each.
The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions: ELISA: 1:4000-1:1600Immunohistochemistry (frozen and paraffin): 1:50-1:300Western Blot: 1:200-1:500, detects a band of ~117kD using E.
coli beta-galactosidase
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：E. coli
Grade：Highly Purified
Purity：Purified by Ion exchange chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes E. coli beta-galactosidase.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ec
Immunogen：Beta galactosidase prepared from E. coli.
