84-1203-84　Von Willebrand Factor (VWF, F8VWF) 1ml　V2700-01K

特徴

• Factor VIII related antigen or von Willebrand factor is a multimeric glycoprotein.
• It has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin.
• von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules.
• It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
• von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms.
• As not all endothelial cells synthesize / store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant.
• Staining for factor VIII related antigen has also been used to measure angiogenesis, an indicator of tumor recurrence.
• Applications:Suitable for use in ELISA, In immunoelectrophoresis, Bi-dimensional Electrophoresis and Radial Immunodiffusion.
• Other applications not tested.
• Recommended Dilution:Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mmdiameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
• Single Radial Immunodiffusion: 1% in the gel
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.