Applications:Suitable for use in ELISA, Immunoelectrophoresis, Single and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 120ul against 2ul serum or equivalent Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and diluted) in 2mm diameter peripheral wells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1ml
Host:goat
Source Antibody:mouse
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:Recognizes mouse transferrin. In immunoelectrophoresis and radial immunodiffusion, a characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Mo
Immunogen:Purified transferrin isolated from pooled mouse serum.