Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in freeform.
Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specificmucosal antibody activity.
A molecule of sIgA is made up of two molecules of IgA, one J chainand one SC (MW 65000).
The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells.
SC also has an affinity for polymeric IgM.
Applications:Suitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P): ~1.6
Excitation: 492nm
Emission: 515nm
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
仕様
Size：1ml
Host：goat
Source Antibody：monkey
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes monkey secretory component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Mk
Immunogen：Purified free monkey secretory component isolated from pooled milk.
