Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form.
Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity.
A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000).
The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells.
SC also has an affinity for polymeric IgM.
Applications:Suitable for use in ELISA, Immunoelectrophoresis, Double Radial Immunodiffusion, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:500-1:1000Immunohistochemistry: 1:20-1:100Immunocytochemistry: 1:20-1:100Optimal dilutions to be determined by the researcher.
Biotin/IgG protein molar ratio (B/P): ~8.6
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：monkey
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes monkey secretory component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon.
Isotype：IgG
Calc Applications Abbrev：E IC IHC
Calc Crossreactivity：Mk
Immunogen：Purified free monkey secretory component isolated from pooled milk.
