Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form.
Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity.
A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000).
The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells.
SC also has an affinity for polymeric IgM.
Applications:Suitable for use in ELISA, Immunoelectrophoresis, Immunoprecipitation and Single and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 2ul against 120ul antiserum Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：monkey
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes bound secretory component (secretory IgA) and free secretory component present in monkey secretions. In immunoelectrophoresis against monkey milk, using a high electroendosmosis agar plate, free SC is precipitated in the alph-2 region. Does not react with other molecular forms of IgA or with any other secretory or plasma protein. Precipitation reactions have been observed with free and bound secretory component in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon.
Isotype：IgG
Calc Applications Abbrev：E IP
Calc Crossreactivity：Mk
Immunogen：Purified free secretory component isolated from pooled rhesus monkey milk
