84-1202-89　Secretory Component 1ml　S0630-01H

特徴

• Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form.
• Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity.
• A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000).
• The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells.
• Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC.
• SC also has an affinity for polymeric IgM.
• Applications:Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
• Other applications not tested.
• Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul serum or equivalentDouble Radial Immunodiffusion: Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.