Ribonuclease A is used to isolate DNA for RNA-free DNA.
Ribonuclease A hydrolyzes RNA at C and U residues, cleaving between the 3’-Phosphate group of a pyrimidine nucleotide and the 5’-hydroxyl group of the adjacent nucleotide, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains (Volkin and Cohn 1953).
The composition and structure of ribonuclease A has been extensively investigated.
The tertiary structure was reported by Kartha, et al.
(1967) and Lin, (1970).
Wang, et al.
(1975) indicate that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA.
The effect of denatured DNA is much greater than that of the native nucleic acid (Sekine et al.
1969).
Applications:Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition.
Other applications have not been tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by solid phase affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, Dextran. Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes Ribonuclease A.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo
Immunogen：Ribonuclease A isolated and purified from bovine pancreas.
