Properdin is a factor of the alternative complement activation pathway.
It has a molecular weight of 222000 and consists of four peptide chains.
Properdin is stabilizing the C3 convertase C3bBb.
The mean concentration in plasma is 20mg/ml.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and Single or Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 120ul against 2ul human serum
Single Radial Immunodiffusion: 1% in the gel
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wellsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. No preservative added. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human Properdin. A single precipitin line is obtained in immunoelectrophoresis, bidimensional electrophoresis and double radial immunodiffusion (Ouchterlony) against normal plasma, which shows a reaction of identity with the precipitated purified properdin. Does not crossreact with any other component of human plasma.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Properdin isolated from pooled human plasma
