Transthyretin is the fastest protein in the immunoelectrophoresis pattern of human serum.
It consists of four identical polypeptide chins with no carbohydrate.
The molecular weight is 55000 and the concentration in normal serum ranges from 100 to 400ug/ml.
It can bind thyroxine and retinal and its concentration is reduced in severe liver diseases.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and single and double radial immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mmdiameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
Single Radial Immunodiffusion: 1% in the gel
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：porcine
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human transthyretin (prealbumin).
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Transthyretin isolated from pooled normal human serum.
