Native plasminogen is a single polypeptide chain, synthesized in the liver.
Its molecular weight has been reported to be 81000 and 92000.
Part of the molecule contains the active serine esterase of plasmin.
On activation it is converted to two polypeptide chains linked by disulphide bridges.
Three different abnormal molecular forms of plasminogen have been described
Normal adult plasma contains 10-20mg/100ml plasminogen.
Different congenital molecular structure abnormalities associated with recurrent thrombosis have been described, but are extremely rare.
In liver disease plasminogen activity may be reduced.
Applications:Suitable for use in Electroimmunodiffusion (Laurell), Immunoelectrophoresis, Double Radial Immunodiffusion, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilution:Electroimmunodiffusion (Laurell): 1-2% agarose gel, depending on the test arrangement
Note: EDTA should be added to the plasma samples and all assay components to stabilize the proteins; to prevent activation of plasminogen in plasma samples epsilonaminocaproic acid may be added
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human Plasminogen. A single precipitin line is obtained in bidimensional electrophoresis, immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) which shows a reaction of full identity with the purified immunogen. No precipitation is obtained with any other plasma protein or with plasminogen-deficient plasma. May also react with plasmin conversion intermediates and with plasmin. Does not crossreact with any other human plasma proteins. Species Crossreactivity: Bovine, canine, rhesus monkey
