Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) hydrolyzes both 3'-5'- phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity.
The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0.
Applications:Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition.
Other applications have not been tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Grade：Highly Purified
Purity：Purified by ammonium sulphate precipitation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes Nuclease P1.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Nuclease P1 isolated and purified from Penicillium citrinum.
