Neuraminidase (sialidase) splits off N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins and are important tools for the study of glycoproteins in protein chemistry and cell biology (Hatton et al.
1973).
Chromatographic purification has been developed in this laboratory based on work of Cassidy et al.
(1965).
Wooley and Gommi (1966) describe the use of this neuraminidase in a method for measuring serotonin receptors.
It is also employed to determine bound N-acetylneuraminic acid in tissues.
Considerable interest has been shown in using neuraminidase inhibitors as possible anti-viral and anti-bacterial agents.
Applications:Suitable for use in Immunoelectrophoresis, RID, ELISA, Western Blot and Inhibition.
Other applications have not been tested.
Recommended Dilutions: Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5mg
Host：rabbit
Grade：Highly Purified
Purity：Purified by ammonium sulphate precipitation and ion exchange chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes Neuraminidase.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Neuraminidase isolated and purified from Athrobacterium ureafaciens.
