Applications:Suitable for use in ELISA, Immunoelectrophoresis and single and double radial immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mmdiameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
Single Radial Immunodiffusion: 1% in the gel
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：porcine
Source Antibody：equine
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes Equine Light Chains (Free and Bound). Reacts with determinants exposed on the surface (SD) and the Fab portion of all intact immunoglobulins, with Fab or F(ab’)2 subunits, and with free light chains of both types; also with determinants exposed only on free light chains but hidden in the intact immunoglobulin molecule (HD or hidden determinants) or Bence Jones proteins. It does not react with any non-Ig protein in horse serum, as tested by immuno- electrophoresis and double radial immunodiffusion and is not kappa or lambda chain specific.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Eq
Immunogen：Purified equine light chains isolated from polyclonal horse IgG.
