Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue.
It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point.
The molecular weight of the monomeric subunit is near 17000.
It is very sensitive to denaturation.
Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation.
Three genetic variants A, B and C have been identified.
They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions.
No immunological differences was found between the variants.
Beta-lactoglobulin can be purified in crystallized form
Allergic reactions of infants to bovine milk proteins are well known.
The actual process by which such reactions develop and their impact on the infant’s health have not been unambiguously established.
Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Applications:Suitable for use in Immunoelectrophoresis and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells
Single Radial Immunodiffusion: 0.5-1% in agar gelOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Specificity：Recognizes bovine beta-Lactoglobulin. Species Crossreactivity: sheep and goat
