In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony, Mancini) against normal plasma, one single precipitin line is obtained.
No precipitation is observed against plasma of patients with high molecular weight kininogen deficiency, but its presence can be demonstrated in normal serum.
In double radial immunodiffusion the precipitin line obtained against normal plasma shows a reaction of full identity with the precipitin line with purified high molecular weight kininogen.
Does not crossreact with any other human plasma proteins.
Applications:Suitable for use in Electroimmunodiffusion, Immunoelectrophoresis and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Electroimmunodiffusion (Laurell): 1-2% with agarose gel
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human high molecular weight kininogen. In double RID a reaction with monkey has been observed.
Isotype：IgG
Calc Applications Abbrev：IP
Calc Crossreactivity：Hu
Immunogen：Highly purified high molecular weight kininogen isolated from pooled human plasma.
