Insulin is a hormone produced by the beta cells of the islets of Langerhans in the pancreas.
It plays an important role in the metabolism of carbohydrate by its effects on the storage of glycogen in the liver, the conversion of carbohydrate to fat, and the oxidation of glucose by the peripheral tissues.
Insulin has a molecular weight of 6000 and contains two peptide chains linked by two disulphide bonds.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Fluorochrome/IgG protein molar ratio (F/P): ~2.7
Excitation: 492nm
Emission: 515nm
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：1ml
Host：guinea pig
Source Antibody：porcine
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
