Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form.
Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity.
A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65000).
The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells.
Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC.
SC also has an affinity for polymeric IgM.
Applications:Suitable for use in ELISA, Western Blot, Dot Blot, Immunocytochemistry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Biotin/IgG protein molar ratio (B/P): ~4.5
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human IgA, Secretory Component. Reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free secretory component is precipitated in the alph-2 region. Does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
Isotype：IgG
Calc Applications Abbrev：DB E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Purified free human secretory component isolated from pooled milk
