84-1200-56　Secretory Component (Free and Bound) 1ml　I1890-31D

特徴

• Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
• The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions.
• In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region.
• The antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein.
• In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in human serum or other body fluids and to determine its concentration.
• Applications:Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
• Other applications not tested.
• Recommended Dilution:Immunoelectrophoresis: 2ul plasma or equivalent against 120ul antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mmdiameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
• Optimal dilutions to be determined by the researcher.
• Precipitin Titer:1:32; tested against pooled normal human milk in agar-block immunodiffusion titration.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.