One of the best studied is the alpha-foetoprotein.
It cab easily be identified immunochemically because of its distinct antigenicity.
In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions.
Its molecular weight is 64000, the carbohydrate content is relatively low.
The concentration of alpha-foetoprotein gradually decreases during foetal growth.
It is not found in normal adult serum, but reappears in most human developing hepatoma.
Applications:Suitable for use in FLISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mmdiameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
Single Radial Immunodiffusion: 1% in the gel
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
Specificity：Recognizes human alpha- Foetoprotein.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：Purified human alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum
