This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen.
This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds.
The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII.
Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and single and double radial immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserumDouble Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mmdiameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameterperipheral wells
Single Radial Immunodiffusion: 1% in the gel
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in PBS, pH 7.2. Preservative free. Reconstitute with 1ml sterile dH2O.
Specificity：Recognizes human Coagulation factor IX.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Highly purified form from fresh plasma concentrates.
