Applications:Suitable for use to sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
Other applications have not been tested.
Recommended Dilutions: Haemolytic complement assays: 1:60 (Haemolytic titre) at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement and 2% RBC, not less than 1:500 when tested with bovine erythrocytes
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:5mg
Host:rabbit
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified by DEAE chromatography.
Form:Supplied as a lyophilized powder. No preservatives added. Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Specificity:Recognizes bovine Erthyrcytes. Homologous proteins of different species frequently share antigenic determinants.
Isotype:IgG
Calc Crossreactivity:Bo
Immunogen:Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization.