Cortisol binding globulin is an alpha-1 glycoprotein with a molecular weight of 55,700.
It is bonding and transporting cortisol in plasma.
The mean concentration in adult serum is about 50ug/ml.
Elevated levels are observed in pregnancy.
Applications:Suitable for use in ELISA, Immunoelectrophoresis, Immunoprecipitation and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 120ul against 2ul human plasma or equivalentDouble Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells
Single Radial Immunodiffusion: 1% in gelOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human Cortisol Binding Globulin (transcortin). In immunoelectrophoresis and radial immunodiffusion against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. Does not react with any other protein component of human serum or plasma.
