Properdin factor B is a heat-labile serum protein active in the alternative complement activation pathway.
It is binding to the C3b component of complement.
The protein has a molecular weight of 92000 and its mean concentration in human plasma is 200ug/ml.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 120ul against 2ul human serum
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in a 3mm diameter center well and 10ul in 3mm diameter peripheral wells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human Properdin Factor B. In immunoelectrophoresis against human EDTA plasma a single precipitin line is obtained in the beta-2 region. Does not react with any other protein component of human serum or plasma. A weak reaction with mouse has been observed indouble radial immunodiffusion.
