The activated protein forms with the complement factors C6-C9 the membrane attack-complex.
Applications:Suitable for use in Indirect Immunofluorescence, ELISA, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Fluorescein/IgG protein molar ratio (F/P): ~2.2
Excitation: 492nm
Emission: 515nm
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human Complement C5. Does not react with any other proteins component of human serum or plasma.
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：C5 isolated and purified from pooled normal human serum.
