C4-binding protein is a plasma protein with a molecular weight of 500000 and consists of 6 chains.
Its concentration in serum is about 250ug/ml.
It is one of the inhibitors of the complement activation system.
The protein combines up to six molecules of C4b at or near the C2 combining site and is thus preventing further association with C2.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent in agarose-plates
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human C4 binding protein. In immunoelectrophoresis and radial immunodiffusion against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. Does not crossreact with any other human plasma proteins.
