C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Applications:Suitable for use in FLISA, Immunofluorescence, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
Excitation: 492nm
Emission: 515nm
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O, dilute further with PBS, pH 7.2.
Specificity：Recognizes rabbit C3, C3c. In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of rabbit serum or plasma.
Isotype：IgG
Calc Applications Abbrev：FLISA IC IF IHC
Calc Crossreactivity：Rb
Immunogen：C3c isolated and purified from pooled normal rabbit serum
