C3 is the most abundant complement protein in rabbit serum.
Its biological function strongly resembles that of C3 in man and other laboratory animal species.
It has a central role in the activation system being common to both pathways.
Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments.
The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can beinteract with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response
The polyclonal antibody is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Consequently the antibody reacts with both native and activated C3.
It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul rabbit plasma or equivalentOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. No preservatives added. Reconstitute with1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes rabbit C3 and C3c. In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of rabbit serum or plasma.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Rb
Immunogen：C3c isolated and purified from pooled normal rabbit serum
