C3 is the most abundant complement protein in mouse serum.
Its biological function strongly resembles that of C3 in man and other laboratory animal species.
It has a central role in the activation system being common to both pathways.
Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments.
The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle; degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.
The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Consequently the antiserum reacts with both native and activated C3.
It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.
Applications:Suitable for use in ELISA, Immunoelectrophoresis, Immunocytochemistry, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:ELISA: 1:500-1:2000Immunohistochemistry: 1:50-1:250Optimal dilutions to be determined by the researcher.
Precipitin Titre:1:16 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5mg
Host：goat
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes native mouse Complement C3, C3c. Does not react with any other protein components of mouse plasma.
Isotype：IgG
Calc Applications Abbrev：E IC IF IHC
Calc Crossreactivity：Mo
Immunogen：C3c isolated and purified from pooled normal mouse serum.
