C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Applications:Suitable for use in Immunoelectrophoresis and ELISA.
Other applications not tested.
Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul guinea pig plasma or equivalent Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：guinea pig
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes guinea pig C3c. In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of mouse serum or plasma.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Gp
Immunogen：C3c isolated and purified from pooled normal guinea pig serum
