C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i.
It iscomposed of an intact beta chain bound to two fragments of the alpha chain.
Applications:Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:500Optimal dilutions to be determined by the researcher.
Biotin/IgG protein molar ratio (B/P): ~4.9
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：monkey
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes monkey C3, C3c. In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Also reacts with fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of monkey serum or plasma. Species Crossreactivity: Old world monkeys (Cercopithecus, Cynomolgus and Baboon), chimpanzee, human
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mk
Immunogen：C3c isolated and purified from pooled normal monkey serum.
