C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Applications:Suitable for use in FLISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Excitation: 554nm
Emission: 573nm
Molar Ratio: ~1.4
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Rhodamine conjugates are sensitive to light.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Rhodamine. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human C3c. In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a singleprecipitin line in the alpha-2 region is obtained. Can also react with fragments C3b, C3bi andsmaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.
Isotype：IgG
Calc Applications Abbrev：E FLISA
Calc Crossreactivity：Hu
Immunogen：C3c isolated and purified from pooled normal human serum.
