C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I.
It is composed of an intact beta chain bound to two fragments of the alpha chain.
Applications:Suitable for use in Immunoelectrophoresis, Dot Blot, Immunocytochemistry, Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months at -20°C after receipt.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Source Antibody：human
Grade：Purified
Purity：Purified.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with Horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human C3c. In IEP against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Can also react with fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. Does not react with any other proteins component of human serum or plasma.
Isotype：IgG
Calc Applications Abbrev：DB E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：C3c isolated and purified from pooled normal human serum.
