84-1198-92　Complement C3c (FITC) 1ml　C7850-14C5

特徴

• Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2.
• Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin.
• C3 is an acute phase reactant.
• Synthesis of C3, a glycoprotein, is induced during acute inflammation.
• The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages.
• A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1,663aa.
• This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
• Applications:Suitable for use in FLISA, Immunoelectrophoresis and single and double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:20- 1:80
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.