84-1198-91　Complement C3, C3c 5mg　C7850-14C3

特徴

• Human Complement component 3, C3 (alternative names include acylation-stimulating protein (ASP) C3 is encoded by gene located 19p13.3-p13.2.
• Because C3, C4, and C5 are strikingly similar suggesting a common evolutionary origin.
• C3 is an acute phase reactant.
• Synthesis of C3, a glycoprotein, is induced during acute inflammation.
• The liver is the main site of synthesis, although small amounts are also produced by activated monocytes and macrophages.
• A single chain precursor (pro-C3) of approximately 200kD is found intracellularly; the cDNA shows that it comprises 1,663aa.
• This is processed by proteolytic cleavage into alpha (~115kD) and beta subunits (~75kD) which in the mature protein are linked by disulfide bonds.
• Applications:Suitable for use in ELISA, Immunoelectrophoresis and single and double Radial Immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration.
• Other applications not tested.
• Recommended Dilution:immunoelectrophoresis: 2ul or equivalent against 120ul antiserum.
• Double radial immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul antiserum in a 3mm diameter centre well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
• Single immunodiffusion: 1% antiserum in agar gel to establish a standard curve
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.