Applications:Suitable for use in ELISA, Immunoelectrophoresis and Radial Immunodiffusion.
Other applications not tested.
Recommended Dilution:Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent in agarose plates.
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells
Single Radial Immunodiffusion: 1% in gelOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity：Recognizes human complement complex. In immunoelectrophoresis and radial immunodiffusion against human plasma a single precipitin line is obtained in the beta-1 region and shows a reaction of full identity with the purified immunogen. Does not react with any other protein component of human serum or plasma.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Complement complex of C3c, C3d and C4 isolated and purified from pooled normal human plasma.
