Applications:Suitable for use in ELISA, Western Blot, Dot Blot, Immunohistochemistry and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:1000-1:20,000Western Blot: 1:1000-1:20,000Immunohistochemistry (Paraffin): 1:1000-1:20,000Immunocytochemistry: 1:1000-1:20,000Dot Blot: 1:1000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1ml
Host:rabbit
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified by ammonium sulfate precipitation and Ion Exchange chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:Recognizes bovine carbonic anhydrase.
Isotype:IgG
Calc Applications Abbrev:DB E IC IHC WB
Calc Crossreactivity:Bo
Immunogen:Carbonic anhydrase isolated and purified from bovine erythrocytes