The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules whichcontain a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues.
At pH 4.5-5.6 itexist as a dimer, above pH 7.0 predominantly as a tetramer.
The monomer has a molecular weight of about 52000.
Applications:Suitable for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Horseradish peroxidase/IgG protein molar ratio (E/P): ~1.3
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：1ml
Host：goat
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Preservative free. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O. Dilute further with PBS, pH 7.2.
Specificity：Recognizes Canavalia ensiformis. Gives one characteristic precipitin line when tested in Immunoelectrophoresis.
