The defined antibody specificity is directed to albumin as tested against bovine sera.
In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin.
The cytochemical grade of allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g.
ELISA), both as capture antibody and detection reagent; in Western blotting.
This product is not pre-diluted.
Applications:Suitable for use in ELISA, Indirect Immunofluorescence, Dot Blot and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:5mg
Host:rabbit
Source Antibody:bovine
Grade:Highly Purified
Purity:Purified by DEAE chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
Specificity:Recognizes bovine Albumin. Species Crossreactivity: feline, canine, goat, guinea pig, hamster, equine, human, monkey, mouse, rat, sheep, porcine. Does not react with chicken, duck, pigeon or rabbit.
Isotype:IgG
Calc Applications Abbrev:DB E IF WB
Calc Crossreactivity:Bo
Immunogen:Highly purified albumin isolated from bovine serum.