Albumin is a stable small polypeptide with a strong antigenicity.
Its molecular weight is about 69000.
It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules.
Applications:Suitable for use in ELISA, Immunohistochemistry, Immunoelectrophoresis, Double Radial Immunodiffusion and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5000-1:15,000Immunohistochemistry: 1:100-1:500Immunocytochemistry: 1:100-1:500Optimal dilutions to be determined by the researcher.
Biotin/IgG protein molar ratio (B/P): ~6.4
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1ml
Host：goat
Source Antibody：rat
Grade：Highly Purified
Purity：Purified by DEAE chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes rat Albumin serum. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. Species Crossreactivity: canine, guinea pig, equine, human, mouse, monkey porcine and rabbit. Does not crossreact with bovine, chicken, goat or sheep.
Isotype：IgG
Calc Applications Abbrev：E IC IHC
Calc Crossreactivity：Ca Eq Gp Hu Mk Mo Po Rb Rt
Immunogen：Highly purified albumin isolated from rat serum
