Applications:Suitable for use in ELISA, Immunoelectrophoresis and Double Radial Immunodiffusion.
Other applications not tested.
Recommended Dilutions:Immunoelectrophoresis: 120ul against 2ul serum, plasma or equivalent Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and serially diluted) in 2mm diameter peripheral wells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1ml
Host:rabbit
Source Antibody:human
Grade:Serum
Purity:Serum
Form:Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:Recognizes human Albumin. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against human serum and the purified albumin. Species Crossreactivity: bovine, canine, goat, guinea pig, equine, monkey, mouse, rat, sheep, porcine. Does not react with chicken or rabbit.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Bo Ca Eq Gt Gp Hu Mk Mo Po Rt Sh
Immunogen:Highly purified albumin isolated from human serum