84-1196-33　UGT1A1 (Glucuronosyltransferase 1A1, UDP-glucuronosyltransferase 1­A1, UDPGT 1­1 and HUG­BR1) 100ug　U1275-27

特徴

• UGT1A1 is a 52-57kD member of the UGT1A subfamily, UGT family of enzymes.
• UGT1A1 is expressed by the liver, and catalyzes the conjugation of glucuronic acid (GA) from UDPGA to lipophilic acceptors such as (anti-cancer) drugs and bilirubin.
• Addition of glucuronic acid increases target solubility and facilitates elimination.
• Mature human UGT1A1 is a 508aa type I transmembrane ER glycoprotein.
• It contains a 465aa luminal domain (aa26-490) plus a 26aa cytoplasmic region.
• The luminal domain is unusual in that aa157-176 are embedded in the ER membrane.
• aa29-444 contain the enzyme active site.
• The cytoplasmic tail appears to mediate noncovalent homodimerization, and heterodimerization with UGT2B.
• The signal sequence aa1-25 is normally cleaved, but a Lys15Arg mutation blocks insertion into the ER membrane.
• There are multiple point mutations that impact enzyme activity.
• One potential splice form is reported that shows a six aa substitution for aa289-533.
• Over aa60-186, human UGT1A1 shares 64% aa identity with mouse UGT1A1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.