Mouse Trem-like transcript 2 protein is a 34kD (predicted for unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins.
Mouse TLT-2 is synthesized as a 329aa precursor that contains a 24aa signal sequence, a 246aa extracellular domain (ECD), a 21aa transmembrane segment, and a 38aa cytoplasmic tail.
Within the ECD are an Ig-like V-type domain aa25-126 and one potential site for N-linked glycosylation.
Mature mouse TLT-2 shares 49aa sequence identity with mature human TLT-2.
Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines.
TLT-2 is also expressed constitutively on CD8+ T cells.
Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 2.5ug labels 10e6 cellsWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Specificity：Recognizes mouse TREML2. Species sequence homology: Recombinant mouse TREML1, rmTREML4, rmTREM1, rmTREM2b, rmTREM3, rmTREM4, and recombinant human TREM2 (1%).
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to aa25-270 from TREML2/TLT-2 expressed in CHO cells (Q2LA85).
