84-1196-29　TINAG (Tubulointerstitial Nephritis Antigen, TIN-Ag) 100ug　T5475-50

特徴

• TIN-Ag (Tubulointerstitial nephritis antigen) is a 48-58kD glycoprotein member of the peptidase C1 family of molecules.
• It is secreted by renal tubule epithelium and (presumably) small intestine columnar epithelium.
• TIN-Ag is an integral component of the renal tubule basement membrane (BM), and appears to promote proper BM matrix organization, block laminin polymerization, and serve as a receptor for the epithelial integrins a3b1 and avb3.
• Epithelial dissociation from the BM is associated with an epithelial-to-mesenchymal transition.
• Human TIN-Ag is synthesized as a 476aa preproprecursor.
• It contains a 19aa signal sequence, a 30aa furin-cleavable prosegment (aa20-49), and a 427aa mature region (aa50-476).
• Within the mature region is an SMB domain (aa61-106), one vWFC domain (aa119-154), and a nonenzymatic peptidase C1A region (aa218-466).
• Multiple splice variants are possible, and potential isoforms may show an alternative start site at aa322, or a combined deletion of aa119-169 plus 209-300, or a combination of an alternative start site at aa19 coupled to a 13aa substitution for aa209-476.
• Over aa20-476, human proTIN-Ag shares 86aa identity with mouse proTIN-Ag; over the mature region (aa50-476), human TIN-Ag shares 88aa identity with mouse TIN-Ag.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.