84-1196-25　SOD3 (Extracellular Superoxide Dismutase [Cu-Zn], EC-SOD) 100ug　S8060-10W

特徴

• Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring, but harmful, superoxide radicals into molecular oxygen and hydrogen peroxide.
• Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30kD.
• Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology.
• SOD3 shares 23%, 17% sequence identity with SOD1 and SOD2, respectively.
• SOD3 shares ~64% sequence homology with mouse and rat SOD3.
• Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution.
• SOD3 is a secretory protein and is synthesized with a putative 18aa signal peptide preceding the 222aa in the mature SOD3.
• SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues.
• SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.